A/C DIAGNOSTICS ENZYMATIC L-Cysteine ASSAY
Assay Procedure of A/C Enzymatic L-Cysteine Assay
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Sample + Working R1 Working R2 Chromogen R3 and R4 Read at OD 660-680nm
or 20 µl ) (200 µl ) (40 µl) (RI 25 µl/RII 15µl)
(37oC for 20 min.) (37oC for 10min.) (37oC for 10min.)
High plasma cysteine is linked to obesity (Plasma concentrations of cysteine, but not other sulfur amino
acids, correlate strongly with fat mass and BMI in men and women).
Also high plasma cysteine is related to cardiovascular disease, metabolic syndrome and cancer.